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Multiplex immunofluorescence (mIF). (A) Images of the H&E staining and (B) the corresponding mIF staining of a representative tissue microarray core from the validation cohort. (C) In the high-magnification view of (B), intratumoral lymphocytes are highlighted (arrow). (B, C) Blue, DAPI; green, pan-cytokeratin; red, α-smooth muscle actin; yellow, CD45.
