FIG. 5.

ConA, ceramide, and MHC I immunofluorescent staining of VZV-infected cells. VZV-infected cells were labeled with Texas red-conjugated ConA (A to C) or Texas red-conjugated BODIPY-ceramide (D to F), fixed, permeabilized, and stained with anti-MHC I MAb and FITC-conjugated anti-mouse IgG antibody. Fluorescent images were overlayed to assess colocalization (C and F). The yellow color represents colocalization of MHC I molecules and the fluorescent marker of the Golgi compartment (F). No colocalization was detected with the fluorescent ER marker (C).