Figure 2.

Temperature-sensitive lethality and cell separation defects in a pmh1-26 strain. (A) Serial dilutions of pmh1⁺ and pmh1-26 strains grown at 28 (left) and 36°C (right). (B) Cell number (measured by Coulter counter) and septation index during time course of incubation at 36°C of wild-type and pmh1-26 cells. (C) Micrographs of pmh1-26 cells fixed and stained with 4,6-diamidino-2-phenylindole and calcofluor after 12 h at 36°C. (D) Number of nuclei per cell as a function of time at 36°C in wild-type (left) and pmh1-26 (right) cells.