Figure 1.

Morphology of cells undergoing the blastospore to filament transition. An overnight culture grown in YEPD medium at 30°C was diluted 1:10 into prewarmed YEPD medium at 30°C or at 37°C in the presence or absence of 10% FCS. Induction time (hrs.) is shown on top. Aliquots of cells were fixed in 4.5% formaldehyde, washed twice with 1 × phosphate-buffered saline, and then visualized by Nomarski optics and photographed under 100× magnification. Please note that the 0-h time point photograph shows cells immediately before induction.