Figure 1.

Specificity of the anti-SLBP antibody for SLBP in intact HeLa cells as assayed by immunofluorescence. (A) HeLa cells transfected with either the C2 control siRNA (left) or the S2 siRNA specific for SLBP (right) were subject to immunostaining with the SLBP antibody. (B) To evaluate the knockdown resulting from the S2 siRNA, Western blot analysis with the SLBP antibody was performed using lysates made from C2 (lanes 1–5) or SLBP (lane 6) siRNA-transfected cells. In lanes 1, 5, and 6, 10 μg of total protein was loaded, whereas in lanes 2, 3, and 4, 5, 2, and 1 μg of total protein was used, respectively. The asterisk marks the migration of the 75-kDa protein that cross-reacts with the SLBP antibody and serves as an internal loading control.