Figure 1.

Physical association between the β₁ and hERG1 proteins in SH-SY5Y neuroblastoma cells. (A) CoIP between β₁ and hERG1 proteins in SH-SY5Y cells cultured in the presence of fetal calf serum (lane serum). CoIP between β₁ integrin subunit and hERG1 protein were performed using anti-β₁ (TS2/16) antibodies (IP: anti-β₁) to IP and anti-hERG1 (C54) (top) or anti-β₁ (RM-12) (bottom) antibodies for Western blot. The lane total lysate refers to a Western blot on total lysate of SH-SY5Y cells. The lane no-IP refers to samples where no primary antibody was added to cell lysates, as described in Materials and Methods. Arrows indicate the main immunoprecipitated bands. (B) CoIP between β₁ and hERG1 in SH-SY5Y cells seeded onto PL or LM. CoIP conditions are the same as in A.