Figure 1.

Schematic representation of a mouse brain slice including the auditory cortex and a multielectrode array (MEA) substrate. (A) Coronal slice sectioning in the mouse brain and brain slices (dotted squares). (B) A brain slice was placed on a two-dimensional (2D) MEA substrate. The six layers (L1–L6) of the cortical laminar structure are also illustrated. (C) Schematic drawing of a coronal slice on the MEA substrate. For example, the tetanic stimulus site (ch. 26) is indicated as one of the black squares (left black square). To monitor the excitability of each brain slice over 40 min, an additional electrode site was selected (ch. 32, right black square), and test stimuli were alternatively applied from the two sites during the experimental session.