Figure 3.

Protocol for Arc catFISH and freezing behavior of mice. (A) The order and duration of context exposures are shown. All groups were trained with exposure to context N followed by a 3-h epoch in their home cage (HC) and then exposure to context S. All mice received shock (^*) on Day 1 in context S except group NS. Mice were tested the next day as shown without any shock. NS, S1, and S2 groups were sacrificed after the second HC epoch on Day 2. (B) Freezing during testing on Day 2. Group SX represents the combined data from groups S1 and S2 (thus n = 8 per genotype). For all figures, levels of significance are indicated as follows: (^*) P < 0.05; (^) P < 0.01; (#) P < 0.001.