. 2001 May;75(10):4907–4911. doi: 10.1128/JVI.75.10.4907-4911.2001

TABLE 1.

Summary of HLA restriction, epitope recognition, and TCR β gene usage of CTL clones obtained from subject 115

CTL clone	Sequence	Epitope/position	HLA restriction	TCR; Vβ gene usage
CTL clone	Sequence	Epitope/position	HLA restriction	Vβ	CDR3	Jβ
115 M21^a	`ERYLKDQQL`	gp41/584–592	B14	4	VKDGA	1.2
115 E15^a	`ERYLKDQQL`	gp41/584–592	B14	4	VEDWGGAS	2.1
115 D87	`ERYLQDQQL`	gp41/584–592	B14	8	ALNRVD	2.1
p175b	`SLYNTVATL`	p17/77–85	A2	5	FDS	2.7

Previously described CTL clone sequences (10, 11). CDR3 region-specific biotin-labeled probes designed for each individual clonotype were 27 to 30 nucleotides in length and spanned the Dβ region with some overlap of the adjacent Vβ and Jβ regions. The diversity region-specific probes used for subject 115 were the following: 115 M21 Db (AGC GTG AAG GAC GGG GCT GGC), 115 E15 Db (AGC GTT GAA GAT TGG GGC GGA GCG AGC TCC), 115 D87 Db (GCT AGT GCC CTC AAC AGG GTC GAT GAG CAG), and 115 p175b.33 Db (TGC GCC AGC AGC TTT GAC AGC GAG CAG TAC). Sequence data for CTL clones 115 D87, 115 p175b, 18030 D23, and 15160 ETA49 are available from GenBank under accession numbers AF272739, AF272740, AF272741, and AF272742.