Fig. 4.

CTSB induces DNA damage and cell cycle arrest in retinoblastoma cells by inhibiting BRCA1. A: The expression of BRCA1 was measured by Western blotting; B: The interaction between CTSB and BRCA1 was verified by coimmunoprecipitation; C: The comet assay was used to measure DNA damage; D: The cell cycle in Y79 cells was analyzed by flow cytometry; E: Western blotting was used to measure the levels of p-BRCA1, BRCA1, CTSB and γH2AX; F: Cell cycle-related protein levels were determined by Western blot analysis. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001vs. the NC or RB group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. the OE-CTSB group