Fig. 5.

CTSB mediates lysosomal stress-induced ferroptosis and autophagy through the STAT3/STING1 pathway. A: The expression of STAT3 was measured by Western blotting; B: Western blotting was used to measure the levels of CTSB, p-STAT3, STAT3, and STING1; C: Ferroptosis-related protein levels were measured by Western blotting; D: Autophagy-related protein levels were measured by Western blotting; E: The content of intracellular MDA was measured by a kit; F: Flow cytometry was used to measure ROS levels; G: Apoptosis was measured by flow cytometry; H: The concentrations of inflammatory factors were measured by ELISA; I: RT‒qPCR was used to measure the expression of lysosomal markers. ^**P < 0.01, ^***P < 0.001 vs. the NC or RB group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. the OE-CTSB group