Fig. 1.

Electrophysiological recording of muscle activity in response to direct stimulation of control and grafted nerve. (A) Representative recordings of CMAPs in control and reinnervated muscle. The i.v. injection of vecuronium completely blocked muscle response to control nerve stimulation but not muscle response to stimulation of grafted nerve (Reinnervated). Next, administration of the AMPA receptor antagonist GYKI 52466 totally abolished the muscle response resistant to vecuronium (Table 1). (B) In these experiments, GYKI 52466 was administrated after the first CMAP recording. GYKI 52466 did not modify the control muscle response but totally prevented the response of reinnervated muscle. Traces are from a representative experiment. The stimulus strength was always adjusted to obtain maximal CMAP amplitude. Measurement of mean ± SD of CMAPs amplitude and area are reported in Table 1.