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. 2005 Jun 3;102(24):8752–8757. doi: 10.1073/pnas.0500530102

Fig. 3.

Fig. 3.

Expression analysis of GluRs in control and reinnervated muscle. (A) RT-PCR analyses of GluR1, GluR2, GluR3, GluR4, and NR1 transcripts in motor end plate of control (C, open bar) and reinnervated (R, black bar) obliquus muscles. The gel-like images produced by Agilent Bioanalyzer 2001 are shown for the coamplification of β-actin and the different GluR transcripts. Histograms show the means of six individual experiments and standard error. For NR1 analysis, the amplification product from rat cerebral cortex (Cx) was used as a positive control. Statistical analysis was performed by using two-tail Student's t test. (*, P < 0.05). An example of electropherogram traces of β-actin/GluR1 coamplification is reported (Bottom Right). Upper and lower markers are used as internal standards to eliminate sample to sample variation, and calculate size and concentration of each PCR product. (B) Western blot analysis of GluR subunits NR1, GluR1, GluR2, GluR3, GluR4, and of β-tubulin in proteins extracts from control (C) and reinnervated muscles (R). (C) Confocal images of control and reinnervated muscle double labeled with antibodies to GluR1 (Cy2, green) and βIII tubulin (Cy3, red). A punctuate GluR1 staining was evident in control sections. A clustering of GluR1 immunofluorescence appeared in reinnervated muscle at the end of a βIII tubulin-positive axon.