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. 2005 Jun 3;102(24):8573–8578. doi: 10.1073/pnas.0503224102

Fig. 2.

Fig. 2.

mTOR activation in normal and Nf1-/- fibroblasts requires active Ras. (A) Ras GTP levels were quantitated by using a pull-down assay in cells expressing either the NF1-GRD or empty vector (Left). GTP-bound Ras, isolated from the pull-down assay (PD), and total Ras protein, present in the total cell extract (TCE), are shown. Western blot analysis of Nf1+/+ and Nf1-/- MEFs expressing the NF1-GRD or empty vector (Right). (B) Western analysis of serum-starved 3T3 fibroblasts expressing either the NF1-GRD or empty vector in response to 200 nM insulin (Top), 6 μM LPA (Middle), or 20 ng/ml PDGF (Bottom) for the indicated times.