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In situ real-time ThT fluorescence assay for monitoring the aggregation kinetics. Panel A refers to 15 μM Aβ incubated at 37 °C under stirring in the absence (grey) and in the presence of 1 mg/mL HLE (orange). Panel B: ThT fluorescence assay for monitoring the aggregation kinetics of 88 μM insulin incubated at 37 °C under vigorous agitation in the absence (grey) and in the presence of 1 mg/mL HLE (orange).
