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β-structure estimation obtained by absorption spectroscopy with CR of 88 μM insulin incubated at 37 °C under vigorous agitation in the absence (grey) and in the presence of 1 mg/mL HLE (orange). Panel A: β-structure estimation obtained as ratio between absorption intensity at λ = 538 nm and at λ = 505 nm. Panel B: absorption spectra obtained at time 0 (continuous line) and after 210 min (dashed line).
