Table 1.

UHPLC-ESI-MS/MS identification of polyphenol compounds detected in ethanolic extract from hop leaves (cv. Cascade) (DE), as in the extract after lyophilisation (LE). The characteristic mass fragments are reported, as the identification method.

Peak number	Compound	Retetion time (min)	[M − H]-	MS2 ion fragments (m/z)	DE	LE	References
1	Dihydrobenzoic acid-O-hexoside	4	315	153-152-109-108	x	x	Chiancone et al. (2022), Martelli et al. (2020)
2	Galloyl-O-hexoside	4,46		169–125	x	x	Chiancone et al. (2022), Martelli et al. (2020)
3	Hulupone	4,89	331	152-168-153	x		Mass Bank Europe, Mongelli et al. (2015),
4	3-Caffaoylquinic acid	5,35	353	191-179-135	x	x	Shibamoto et al. (2009)
5	Dihydroxybenzoyl-hexose-pentose	5,54	447	152-163-108	x	x	Varga et al. (2020),
6	5-Caffaoylquinic acid	5,87	353	191-173-135	x	x	Shibamoto et al. (2009)
7	Sinapic acid-O-hexoside I	5,94	385	223-208-179-164-149-121	x	x	Murashige et al. (1962), Chiancone et al. (2022)
8	Luteolin dihexoside	6,11	609	285–447	x	x	Lin and Harnly (2010)
9	Benzyl alcohol-hexose-pentose	6,17	407	401-269-161	x	x	Varga et al. (2020),
10	Sinapic acid-O-hexoside II	6,25	385	223-208-179-164-149-121	x	x	Murashige et al. (1962), Chiancone et al. (2022)
11	Coumaroylquinic acid III	6,43	337	173-191-163-119	x	x	Mongelli et al. (2015)
12	Catechine	6,63	289	245	x		Choi et al. (2018), Mass Bank Europe, Mongelli et al. (2015)
13	Rutin (Quercetin-3-O-rutinoside)	6,72	609	300-301-255	x	x	Standard compound
14	Kampferol-O-rutinoside I	7	593	447-389-285-284-255	x	x	Chiancone et al. (2022), Nionelli et al. (2018),
15	Xanthoumol	11,32	353	295-189-133-119	x		Standard compound
16	Cohumulone	13,08	347	278-235-223-209-207-195-194-193	x	x	Chiancone et al. (2022), MoNA – Mass Bank of North America, Martelli et al. (2020)
17	Humulone/Adhumulone	13,57	361	292–249	x		Principe et al. (2014), Pub Chem, Helmja et al. (2007)
18	Post-lupulone	14,32	385	273	x		Chiancone et al. (2022), Principe et al. (2014), Helmja et al. (2007)
19	Colupulone	15,45	399	287-275-219-207	x	x	Chiancone et al. (2022), Mass Bank Europe, Principe et al. (2014)
20	Lupulone	16,06	413	301-289-369	x	x	Standard compound