Figure 2.

Fabrication of polycarbonate devices using machine milling and chloroform‐assisted bonding. A) Manufacturing process of polycarbonate chips. Polycarbonate slabs are milled using a Computer Numerical Control (CNC) milling machine and tubing interfaces are adapted for fittings using a manual thread tapper. Components are assembled using vapor or liquid chloroform deposition followed by application of heat and/or pressure. CNC, heat press and thread tapper images are reproduced from Minitech Machinery Corp., Rosin Technologies LLC. and Vertex Machinery Work Co., respectively; B) Gut chip layers and fully assembled gut chip; C) Single channel tumor chip (SCTC) layers and assembled SCTC chip; D) Quad channel tumor chip (QuadTC) layers and assembled QuadTC; E) Absorption and adsorption by PDMS and polycarbonate following 2 h dye incubation and washing by PBS. Strong residual fluorescence only on PDMS channel surfaces.