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. 2005 Mar 17;24(7):1418–1429. doi: 10.1038/sj.emboj.7600604

Figure 2.

Figure 2

Differential effects of suvh1 and suvh2 mutations on histone and DNA methylation. (A, B) Immunohistochemical staining of wild-type, suvh1 and suvh2 interphase nuclei with antibodies recognizing specific histone and DNA methylation marks. In suvh2, but not in suvh1, mono- and dimethyl H3K9, mono- and dimethyl H3K27, monomethyl H4K20 and 5-methylcytosine (heterochromatic marks) are significantly reduced (A). No effects are found on the trimethyl H3K9, trimethyl H3K27, di- and trimethyl H3K36 and di- and trimethyl H4K20 euchromatic marks (B). (C) Western analysis of nuclear extracts of wild-type, suvh1 and suvh2 mutant plants. Only in suvh2 significant reduction of mono- and dimethyl H3K9 and monomethyl H3K27 is found. (D) In vitro recombinant SUVH2 shows H3 and H4 HMTase activity in assays with reconstituted nucleosomes.