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. 2005 Mar 17;24(7):1406–1417. doi: 10.1038/sj.emboj.7600609

Figure 5.

Figure 5

HDACs, SNF2h, and MCMs colocalize at OriP. (A) Immunoprecipitates (IP) of EBNA1, SNF2h, HDAC2, or IgG were analyzed by immunoblotting (IB) with antibodies specific for SNF2h, HDAC2, or EBNA1 as indicated. (B) ChIP assays with antibodies specific for EBNA1, HDAC1, or HDAC2 were used with extracts from asynchronous (A), serum-starved (G0), mimosine-arrested (G1), or colchicine-arrested (M) Raji cells and assayed for OriP (left panel) or cellular GAPDH (right panel) DNA. (C) G1-arrested Raji cells were subject to double ChIP assay with first-round SNF2h, followed by second-round ChIP with antibodies to SNF2h, HDAC2, MCM3, or control IgG.