Figure 7.

Time course of actinomycin D-induced release of AIF-GFP, cytochrome c, and smac/DIABLO as revealed by fluorescence microscopy. HeLa cells expressing AIF-GFP were treated with actinomycin D in the presence of zVAD-fmk. At the indicated time points, the cells were examined by confocal microscopy for GFP (AIF-GFP) together with immunofluorescence microscopy using antibodies against cytochrome c (left panel) or Smac/DIABLO (right panel). Note that cytochrome c and smac/DIABLO, but not AIF-GFP, were efficiently released to the cytoplasm after 4 h incubation.