FIG. 3.
Maturation-dependent transduction is independent of transgene promoter and dNTP concentration. (A) MDMs were transduced as for Fig. 1A at an MOI of 10 with CMVΔR8.9(VSV-G) packaged vector with eGFP driven from either CMV, human β-actin, human EF1α, or RSV promoters. Cells were analyzed for eGFP expression 7 days posttransduction. (B) CMVΔR8.9(VSV-G) or MLV(VSV-G) vector stocks were treated for 1 h prior to infection with 0, 5, 10, or 20 μM concentrations of dNTPs as shown in parentheses and then used to infect MDMs at an MOI of 10 at various days postisolation. Cells were analyzed for eGFP expression 7 days posttransduction.