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. 2005 Mar 24;24(8):1546–1556. doi: 10.1038/sj.emboj.7600592

Figure 1.

Figure 1

Time course of caspase induction by Ad HA-BIK. (A) H1299 cells stably transfected with vector or vector expressing HA-BCL-2b5 were infected with Ad HA-BIK for the indicated times, after which caspase activity was measured using the fluorescent substrate DEVD-AMC. The results of three independent determinations±s.d. are shown. (B) Expression of BIK in Ad HA-BIK-infected cells. Cells were treated as in (A), proteins were resolved by SDS–PAGE and probed with antibodies against BIK and actin (as a loading control).