Figure 7.

Analysis of liver glycogen synthase activity and glycogen levels in the knockin mice. (A) The indicated mice were fasted overnight and injected intraperitoneally with glucose (2 mg/g) or saline solution (for the 0 min time point), to anaesthetised mice. At the indicated time (10 min for saline control), the liver was rapidly extracted and snap frozen in liquid nitrogen. Glycogen synthase activity was measured in the absence and presence of G6P. The data are presented as the mean±s.e.m. for liver isolated from three mice each assayed ±G6P in duplicate. Liver extracts from the mice were immunoblotted with the indicated antibodies. (B) The WT and knockin mice were fasted overnight for 16 h (Fast) or fed ab libitum (Fed), and liver glycogen content quantified as μmol of glycosyl units per gram of liver. The data are presented as the mean±s.e.m. for liver isolated from six mice in each group each assayed in triplicate.