Figure 6.
Copper ions do not impact the interaction between VjbR and virB promoter. (A) EMSA of His-VjbR and the virB promoter region. His-tag was incubated with the fragment as a negative control. Results are representative of at least three independent experiments. (B) Absorption spectra of VjbR (50 μg/mL) after incubation with different concentrations of Cu (II). (C) Interactions between ArsR2 protein and Cu (II) by MST. Kd represents the dissociation constant. (D) The effect of Cu (II) on DNA binding activity of VjbR. The virB promoter region probe was incubated with VjbR (2 µg) in the presence or absence of Cu (II). Results are representative of at least three independent experiments.