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. 2005 Mar 31;24(8):1584–1595. doi: 10.1038/sj.emboj.7600636

Figure 4.

Figure 4

The ski mutations have minimal effect on nonstopHIS3 expression. (A) Yeast strains (WT, W303; ski2Δ, YIT888; ski3Δ, YIT890; ski7Δ, YIT929) were transformed with pIT709 (pHIS3-His6, left panel) or pIT711 (phis3-His6-ns, right panel). Transformants were streaked on SC-His plate and incubated for 3 days at 30°C. (B) Sample preparation and hybridization were performed as described for Figure 1C. Lanes 1 and 2: W303; lanes 3 and 4: YIT888 (ski2Δ); lanes 5 and 6: YIT890 (ski3Δ); lanes 7 and 8: YIT929 (ski7Δ). Cells were transformed with pIT709 (pHIS3-His6, odd lanes) or pIT711 (phis3-His6-ns, even lanes). (C) Yeast cells described in (B) were grown on SC-Leu medium. Protein separation and Western analysis were performed as described for Figure 2A.