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. 2024 Sep 11;15:1440885. doi: 10.3389/fpls.2024.1440885

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Copyright © 2024 Lampugnani, Persson and van de Meene

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localisation of APEX2-AtCESA6 and NaARADL1-APEX2 constructs using TEM. (A) The control construct 35S:APEX2 with no electron dense labelling adjacent to the cell wall (cw). (B) The APEX2-AtCESA6 sample with electron dense labelling at a comparative region (white arrows). (C–E) Further labelling of the APEX2-AtCESA6 construct in vesicles (v), adjacent to the cell wall (white arrows), and vesicles with what appears to be a translucent centre with electron dense labelling around the outside (blue arrows). (F–H) The NaARADL1-APEX2 labelling showed electron dense labelling in the trans-Golgi, the TGN and in vesicles in the cytoplasm (red arrows). Multiple, non-labelled vesicles (black arrows) were observed in the same region as NaARADL1-APEX2 positive vesicles in a possible SVC. cw, Cell wall; c, cytoplasm; vac, vacuole; v, vesicle; g, Golgi Apparatus; tgn, trans-Golgi network; svc, secretory vesicle cluster. Scale bars = 100 nm.