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. 2024 Sep 11;15:1440885. doi: 10.3389/fpls.2024.1440885

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Copyright © 2024 Lampugnani, Persson and van de Meene

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localisation of APEX2-AtCESA6 and pectin antibodies using TEM. (A, B) Both the JIM5 (A) and JIM7 (B) antibodies immunogold labelled the cell wall (orange arrows) and the APEX2 densities adjacent to the plasma membrane (this immunogold shown with white arrows) in the APEX2-AtCESA6 line. (C, D) The GA were found to have both JIM5 (C) and JIM7 (D) antibody labelling in the trans-Golgi cisternae (red arrows). (E, F) Immunogold labelling was also observed in secretory vesicles clusters (E) and in vesicles (green arrows) adjacent to APEX2-positive vesicles (blue arrows) (D, F). cw, Cell wall; c, cytoplasm; v, vesicle; g, Golgi Apparatus. Scale bars = 100 nm.