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. 2005 Mar 31;24(8):1621–1633. doi: 10.1038/sj.emboj.7600641

Figure 2.

Figure 2

Bub1 depletion leads to errors in chromosome congression. (A) Successive frames every 3 min from live-cell movies of H2B-GFP-expressing HeLa cells transfected with siRNA as indicated. Scale bar: 10 μm. (B) Fraction of cells at T=18 min with uncongressed chromosomes in control and Bub1- and Mad1-depleted cells as determined visually. (C) Fraction of cells with uncongressed chromosomes at indicated time points after NBD in control and Bub1-depleted cells arrested at the metaphase–anaphase transition by MG132 treatment (see Materials and methods for details). (D) Fraction of cells with mitotic errors in anaphase. Green denotes morphologically normal anaphase, red lagging chromosomes, purple failure to divide into two chromosome masses and white sustained mitotic arrest at T=120 min. Error bars represent s.e.m. from four or more independent experiments.