Table 6.
The Composition of Ovarian Cancer Organoid Medium.
| Product | Current study | Kopper et al. 30 | Maenhoudt et al. 33 | Nanki et al. 13 |
|---|---|---|---|---|
| DMEM/F12 | Yes | Yes | Yes | Yes |
| L-glutamine | 1x | 1x | 1x | 1x |
| Penicillin/streptomycin | 1x | 0.2% primocin | 1x | 200 U/mL |
| A83-01 | 5 μM | 0.5 μM | 0.25–0.5 μM | 0.5 μM |
| Nicotinamide | 5 mM | 10 mM | 1–5 mM | / |
| N2 | / | / | 1x | / |
| B27+vitamin A | 1x | 1x | 1x | 1x |
| N-acetylcysteine | 1.25 mM | 1.25 mM | 1.25 mM | 1 mM |
| Estradiol | 100 nM | 100 nM | 10 nM | / |
| p38i (SB203580) | / | / | 1–10 μM | / |
| EGF | 50 ng/mL | 5 ng/mL | 50 ng/mL | / |
| bFGF | / | / | 2 ng/mL | 50 ng/mL |
| FGF10 | 100 ng/mL | 10 ng/mL | 0–10 ng/mL | / |
| Noggin | 100 ng/mL | 1% | 10% | 100 ng/mL |
| RSPO1 | 250 ng/mL | 10% | 20%, 50 ng/mL | 1 µg/mL |
| IGF1 | / | / | 0–20 ng/mL | 100 ng/mL |
| HGF | / | / | 0–10 ng/mL | / |
| Heregulinβ-1 | 37.5 ng/mL | 37.5 ng/mL | 50 ng/mL | / |
| WNT3A | 50 ng/mL | 20% | / | 20% |
| Forskolin | 10 μM | 10 μM | / | / |
| Leu15-Gastrin I | / | / | / | 10 nM |
| Hydrocortisone | 500 ng/mL | 500 ng/mL | / | / |
| Y27632 | 10 μM | 5 μM | 10 μM | 10 μM |