Table 2.
Strains and plasmids used in this study
| Nomenclature | Genotype | Reference |
|---|---|---|
| FB1 | a1b1 | Banuett and Herskowitz (1989) |
| FB2 | a2b2 | Banuett and Herskowitz (1989) |
| FB1GT | a1b2/pGFPTub1 | Steinberg et al (2001) |
| FB1YT1-H4C | a1b1/pYFPTub1/pH4CFP | This study |
| FB2nRFP3 | a2b2/pC_NLS3xRFP | This study |
| FB1ERY-CT1 | a1b1/pERYFP/pCFPTub1 | This study |
| FB2EG | a2b2/pERGFP | Adamikova et al (2004) |
| FB1ERY-H4C | a1b1/pERYFP/pH4CFP | This study |
| FB2nRFP-H4G | a2b2/pC_NLS3xRFP/pH4GFP | This study |
| FB2nRFP-ERG | a2b2/pC_NLS3xRFP/pN_ERGFP | This study |
| FB2nRFP-GT1 | a2b2/pH_NLS3xRFP/pGFPTub1 | This study |
| FB2nRFP-T2G | a2b2/pH_NLS3xRFP/pTub2GFP | This study |
| FB2G2Dyn1-RT1 | a2b2Pdyn1-2xgfp-dyn1, hygR/pRFPTub1 | This study |
| FB1rDyn2 | a1b1Pcrg-dyn2, bleR | Wedlich-Söldner et al (2002a) |
| FB1rDyn2ERY-CT1 | a1b1Pcrg-dyn2, bleR/pERYFP/pCFPTub1 | This study |
| FB1ΔRas1-ERY-CT1 | a1b1Δras3R/pERYFP/pCFPTub1 | This study |
| pYFPTub1 | Potef-yfp-tub1, cbxR | This study |
| pH4CFP | Potef-h4-cfp, hygR | This study |
| pH_NLS3xRFP | Potef-gal4s-mrfp-mrfp-mrfp, hygR | This study |
| pC_NLS3xRFP | Potef-gal4s-mrfp-mrfp-mrfp, cbxR | This study |
| PERYFP | Potef-cals-yfp-HDEL, cbxR | This study |
| pCFPTub1 | Potef-cfp-tub1, hygR | Wedlich-Söldner et al (2002b) |
| PERGFP | Potef-cals-gfp-HDEL, cbxR | Wedlich-Söldner et al (2002a) |
| pN_ERGFP | Potef-cals-gfp-HDEL, natR | This study |
| pH4GFP | Potef-h4-gfp, hygR | This study |
| pGFPTub1 | Potef-gfp-tub1, cbxR | Steinberg et al (2001) |
| pTub2GFP | Potef-tub2-gfp, cbxR | Straube et al (2003) |
| pRFPTub1 |
Potef-mrfp-tub1, cbxR |
This study |
| a, b, mating type genes; P, promoter; -, fusion; hygR, hygromycin resistance; bleR, phleomycin resistance; cbxR, carboxin resistance; /, ectopically integrated; tub1, α-tubulin; tub2, γ-tubulin; h4, histone 4; Dyn1/Dyn2, N- and C-terminal half of the dynein heavy chain; gfp, enhanced green flourescent protein; yfp/cfp, yellow-shifted/cyan-shifted fluorescent protein; mrfp, monomeric red fluorecent protein; calS, signal sequence of calreticulin from rabbit (nt 1–51); HDEL, ER retention signal; gal4s, nuclear localization signal of the GAL-4 DNA binding domain from pC-ACT1 (Clontech). | ||