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. 2005 Apr 14;24(9):1696–1705. doi: 10.1038/sj.emboj.7600648

Figure 5.

Figure 5

METTL1 is phosphorylated in mouse ES cells expressing wild-type PDK1 or PDK1[L155E], but not in PDK1 null cells. Mouse ES cells expressing PDK1[+/+], PDK1[L155E/L155E] or PDK1[−/−] were serum starved for 4 h, incubated for 20 min without (−) or with (+) 100 nM wortmannin, and then stimulated for a further 15 min with 20 ng/ml IGF-1. METTL1 was immunoprecipitated from 2.5 mg cell lysate protein using the antibody raised against mouse METTL1 residues 258–268, denatured in SDS, subjected to SDS–PAGE and, after transfer to Immobilon-P membranes, immunoblotted as in panel A.