Nonsynonymous variation of mitochondrial genome in Spodoptera frugiperda. A) dotted boxes represent number and sites of nonsynonymous variation, blue font represents FAW‐mC strain, whereas red font represents FAW‐mR strain. B) representative TEM images of mitochondrial morphology in flight muscle tissues. The boxed areas were enlarged in the bottom panels. Scale bars, 1 µm (5000×, bottom) and 5 µm (1700×, top). C) percentage of relaxed mitochondria in flight muscle tissues of FAW‐mC and FAW‐mR strain. D) relative number of mitochondria between FAW‐mC and FAW‐mR strains. E, comparison of ATP content between FAW‐mC and FAW‐mR strains (n = 8). Student's t‐test were performed for the analysis of (C), (D), and (E) figures. “*” “**” “*****” indicate significant differences at P < 0.05, P < 0.01, P < 0.0001.