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. 2005 May 5;24(10):1874–1885. doi: 10.1038/sj.emboj.7600659

Figure 5.

Figure 5

Inhibition of auxin responses in the root by shy2-2, but not by bdl. (A) Gravitropic response of pSHY2∷SHY2, pSHY2∷shy2-2, pSHY2∷BDL and pSHY2∷bdl seedlings after reorientation by 90° (data from two to five independent transgenic lines for each genotype). The percentages represent the fraction of seedlings (numbers analyzed in parentheses) with normal gravitropic response. (B) Inhibition of root growth by 2,4-D. Root length (±s.d.) was measured after 3 days of vertical growth on medium with (black bars) or without (white bars) 0.1 μM 2,4-D. Growth is represented as percentage of root length in wild type (COL) on control media. (C) Western blots of protein extracts from IAA-treated (10 μM for 5 h) or untreated roots of pSHY2∷SHY2, pSHY2∷shy2-2, pSHY2∷BDL and pSHY2∷bdl seedlings. Asterisks: unspecific crossreaction as loading control. Note that SHY2 or BDL accumulation is induced by auxin in pSHY2∷SHY2, pSHY2∷BDL and pSHY2∷bdl, but not in pSHY2∷shy2-2. (D) GUS activity in root tips of F1 seedlings from crosses between hemizygous pSHY2∷shy2-2 or pSHY2∷bdl lines and homozygous pSHY2∷GUS or pDR5(7x)∷GUS lines. Controls (WT) are wild-type segregants from the same cross. −IAA, untreated; +IAA, 10 μM IAA for 5 h. GUS staining time was 3 h for untreated and 1.5 h for IAA-treated roots.