Figure 3.

RAD26 inactivation abolishes strand-specific repair and sensitizes cells in G1. rad26Δ cells were synchronized in G1 or G2/M, UV-challenged and then reincubated for repair. (A) Autoradiograms, the legends are as in Figure 1. (B) Quantitative analysis, performed as described in Figure 1D. Dashed lines represent quantification results from Figure 1D (G2/M) that were included for comparison. (C) Survival curves. Synchronized WT and rad26Δ cells were UV-irradiated with increasing UV fluences, and the percentage of surviving cells corresponding to each UV fluence was determined. Each error bar represents the standard deviation of three different experiments.