FIG. 2.
Photograph of electrophoretically separated cell lysates reacted with polyclonal rabbit antiserum to a GST-US3 fusion protein. Replicate 25-cm2 flask cultures of HEp-2 cells were mock infected or infected with 100 PFU of HSV-1(F) per cell for 2 h or 10 PFU of HSV-1(F) or R7041 per cell and, where indicated, coincubated with Bac-α22 or Bac-US3 for 18 h. Cultures were harvested, solubilized, electrophoretically separated on denaturing gels, transferred to a nitrocellulose sheet, and reacted with rabbit antiserum to a GST-US3 fusion protein.