FIG. 5.
Photograph of electrophoretically separated cell lysates reacted with polyclonal antibody to caspase-3. Replicate 25-cm2 flask cultures of HEp-2 cells were either mock infected or infected with d120 and, where indicated, coinfected with Bac-US3 at the indicated times relative to d120 infection. Cultures were harvested 18 h after infection, solubilized, electrophoretically separated on denaturing gels, electrically transferred to a nitrocellulose sheet, and reacted with rabbit antiserum to caspase-3.