Figure 3.
Sustained IOP reduction with subconjunctival ssAAV2-STC-1-FLAG in FP receptor knockout mice. Wild-type littermate control mice (n = 4) and FP receptor knockout mice (n = 5) were injected subconjunctivally with ssAAV2-STC-1-FLAG (2μL; 6 × 109 VG) in 1 eye and the same volume of PBS in the fellow eye. Significant, sustained IOP lowering was seen in the eye injected with ssAAV2-STC-1-FLAG in both wild-type and FP receptor knockout mice starting in week 1 and persisting through week 4. Of note, there was no significant difference in IOP between treated eyes of wild-type and FP receptor knockout mice. FP = prostaglandin F; IOP = intraocular pressure; PBS = phosphate-buffered saline; ssAAV2-STC-1-FLAG = single-stranded, adeno-associated virus, serotype 2, engineered to express stanniocalcin-1 with a FLAG tag; VG = viral genome.