Figure 4.
Subconjunctivally injected ssAAV2-STC-1 without the FLAG tag reduced IOP in a sustained fashion. After baseline IOP measurements, ssAAV2-STC-1 without a FLAG tag (2μL; 6 × 109 VG) was subconjunctivally injected in 1 eye and ssAAV2-GFP (2μL; 6 × 109 VG) was subconjunctivally injected into the fellow eye of 3-month-old C57BL/6J mice (n = 8). Significant sustained IOP lowering was seen in the eye injected with ssAAV2-STC-1 until the experiment was ended. GFP = green fluorescent protein; IOP = intraocular pressure; ssAAV2-STC-1 = single-stranded, adeno-associated virus, serotype 2, engineered to express stanniocalcin-1; VG = viral genome.