Figure 1.

Setup for RNA transfer

(A) Materials and equipment needed before starting. The 1× SSC buffer and gel are not shown. Weight 1 is a protein gel tank filled with water and used as a weight. Weight 2 is a tray that we put in between the plastic wrap and the protein gel tank so the weight can be evenly distributed across the setup.

(B) Step by step picture showing how to assemble the transfer setup (in sequence from 1–9).

(C) Cartoon figure illustrating the setup.