Table 3.

Proteinase K and DBCO-biotin mixture

Reagent	Final concentration	Amount
RNA	N/A	5 μg–15 μg
Tris pH 7.5(200 mM, diluted with RNase-free Water from 2 M Tris-HCl Solution, pH 7.5)	11.1 mM	0.75 μL
Proteinase K Solution (20 mg/mL)	1.1 mg/mL	0.75 μL
UltraPure DNase/RNase-Free Distilled Water	N/A	add to 13.5 μL
Total	N/A	13.5 μL