Figure 2.

Bulk RNA-seq identifies the enrichment of cytotoxic pathways and the absence of myeloid traits in excellent donors

(A) Heatmap showing the hierarchical clustering based on the 1,000 most variable genes, analyzed from 10 donors on day 35. For each donor, single or two biological replicates were used (a and b). (B) Volcano plot of differentially expressed genes in excellent vs. good donors. (C and D) Ten most enriched pathways in excellent and good donors, using in (C) the differentially expressed genes and the GO Biological Process database and in (D) the normalized gene counts and the KEGG database. Significant enrichment threshold was considered as NES p < 0.05 (colored bars) and FDR < 0.25 (bars with diagonal pattern). (E) Heatmap showing the genes from the NK cell-mediated cytotoxicity KEGG pathway that contribute to pathway enrichment in excellent donors. Excellent and good donors are labeled with different colors. DE, differentially expressed; GO BP, Gene Ontology Biological Process; KEGG, Kyoto Encyclopedia of Genes and Genomes; NES, normalized enrichment score; FDR, false discovery rate.