Table 1.
Overview of antibodies/stains used for phenotypic characterization of NK cells and their progenitors during ex vivo cell culture
| Antibody | Fluorochrome | Clone | Cat. no. | Supplier |
|---|---|---|---|---|
| Brilliant staining buffer plus (BSBP) | – | – | 566385 | BD Biosciences |
| ViaKrome | IR885 | – | C36628 | Beckman Coulter |
| CD56 | APC-Fire750 | QA17A16 | 392408 | BioLegend |
| CD3 | PerCPCy5.5 | SK7 | 344808 | BioLegend |
| CD14 | PerCPCy5.5 | HCD14 | 325622 | BioLegend |
| CD15 | PerCPCy5.5 | HI98 | 301922 | BioLegend |
| CD19 | PerCPCy5.5 | HIB19 | 302230 | BioLegend |
| CD16 | BV421 | 3G8 | 302038 | BioLegend |
| NKp30 | BV605 | P30-15 | 325234 | BioLegend |
| NKp46 | BV785 | 9E2 | 331946 | BioLegend |
| DNAM-1 | PE | DX11 | 559789 | BD Biosciences |
| NKp44 | PE-Cy7 | P44-8 | 325116 | BioLegend |
| NKG2D CD159D | APC | 1D11 | 320808 | BioLegend |
| LFA-1 | BV421 | M24 | 363408 | BioLegend |
| CRACC | BV605 | 235614 | 235614 | BD Biosciences |
| CD94 | BV786 | HP-3D9 | 743953 | BD Biosciences |
| NKG2A CD159A | PE | Z199 | IM3291U | Beckman Coulter |
| NKG2C CD159C | PE-Vio770 | REA205 | 130-120-449 | Miltenyi |
| SLAMF6 CD352/NTBA | APC | REA339 | 130-105-543 | Miltenyi |
| CD25 | BV421 | BC96 | 302630 | BioLegend |
| PD-1 | BV605 | NAT105 | 367426 | BioLegend |
| CD69 | BV785 | FN50 | 310932 | BioLegend |
| TIM-3 | PE | F38-2E2 | 345006 | BioLegend |
| FAS-L | PE-Cy7 | Nok-1 | 306418 | BioLegend |
| TRAIL | APC | RIK-2 | 308210 | BioLegend |
| CD96 | BV421 | 6F9 | 742794 | BD biosciences |
| 2B4 | BV605 | C1.7 | 329536 | BioLegend |
| CD2 | BV785 | RPA-2.10 | 300234 | BioLegend |
| SIGLEC7 | PE | 6–434 | 339204 | BioLegend |
| KIR2D | PE-Vio770 | REA1042 | 130-117-483 | Miltenyi |
| KIR3DL1/L2 | PE-Vio770 | REA970 | 130-116-180 | Miltenyi |
| CD161 | APC | HP-3G10 | 339912 | BioLegend |
| CXCR4 | BV421 | 12G5 | 306518 | BioLegend |
| CCR6 | BV605 | G034E3 | 353420 | BioLegend |
| CD62L | BV785 | DREG-56 | 304830 | BioLegend |
| CD57 | PE | QA17A04 | 393308 | BioLegend |
| CCR7 | PE-Cy7 | G043H7 | 353226 | BioLegend |
| CXCR3 | APC | G025H7 | 353708 | BioLegend |