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Expression of bovine and porcine IFN-α or -β in E. coli. Lysates of BL21(DE3) cells containing bovine and porcine pET-IFN-α or -β (bIFN-α or -β and pIFN-α or -β), respectively, were collected at 0, 1.5, or 3 h after IPTG induction, run on an SDS-PAGE (15% acrylamide) and stained with Coomassie blue. Arrows indicate induced IFN proteins. Lanes M, protein molecular mass markers in kilodaltons.
