FIG. 4.

Synthesis of FMDV full-length RNA and protein. IBRS2 cells were treated for 16 h with supernatant from A12-LLV2-infected PK cells containing IFN-α/β (+) or mock-infected PK cells (−) and infected at an MOI of 10 with A12-IC virus. (A) RNA was harvested at 1, 2, and 3 hpi and run on a 1% formaldehyde agarose gel. RNA was transferred to a nylon membrane and hybridized to a peroxidase-labeled full-length FMDV probe, and the signal was detected using a chemiluminescent technique. The arrow indicates full-length FMDV RNA. (B) [³⁵S]methionine-labeled proteins were harvested at 1.5, 2, 2.5, 3, 3.5, and 4 h postinoculation, immunoprecipitated using a polyclonal antibody against FMDV nonstructural protein 3D, and analyzed on an SDS-PAGE (15% acrylamide). Arrows indicate 3D and precursors. Lane c, A12-IC-infected IBRS2 lysate immunoprecipitated with 3D antiserum.