FIG. 1.
Association of Vif with protease-treated virions. (A) NL4-3 virus was produced in cells as indicated and treated with subtilisin. Two aliquots (the second aliquot is a twofold amount of the first) of virus lysates from each producing line were resolved on SDS-PAGE. (B) KS283 virus produced from H9 and MT-2 cells was resolved on SDS-PAGE at a similar p24CA content. Vif was detected in immunoblotting with anti-Vif rabbit antiserum (bottom panels), and Gag was detected by reprobing of stripped membranes with anti-CA monoclonal antibody (top panels).