Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Association of HA-Vif and HA-Vpr with wild-type (wt) or Δ350-362 Gag (Δ) VLPs. Gag was coexpressed with HA-Vif or HA-Vpr in 293T cells, and VLPs released to culture supernatants were collected by ultracentrifugation, with or without detergent treatment. Lysates of sedimented VLPs were analyzed with anti-HA monoclonal antibody for HA-Vif and HA-Vpr (bottom panels) and Gag was analyzed by reprobing of stripped membranes with anti-CA monoclonal antibody (top panels).
