FIG. 7.
Presence of anti-V3 loop antibodies in sera collected from macaques immunized with the modified ΔV2 gp140 immunogen. The development of anti-V3 loop antibodies was determined by ELISA using the V3 loop peptide derived from the SF162/SF162ΔV2 envelope. (A) We first examined whether the captured V3 loop peptide interacts with specific anti-V3 loop MAbs recognizing linear (447D) and conformational (391-95D) V3 loop epitopes. (B) We next determined the titer of anti-V3 loop antibodies present in sera collected 2 and 4 weeks following the 1st and 2nd boosts from the two vaccinated animals. As a comparison, we also include the titers of total antienvelope antibodies present in the same sera.