TABLE 1.
Generation of cross-reactive neutralizing antibodies in rabbits
| gp140 | Animal | Specific neutralizationa
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 91US054
|
92US657
|
92US660
|
92HT593
|
91US056
|
92US714
|
||||||||
| 50% | 80% | 50% | 80% | 50% | 80% | 50% | 80% | 50% | 80% | 50% | 80% | ||
| Unmodified SF162 | A1 | − | − | − | − | − | − | − | − | − | − | − | − |
| A2 | − | − | − | − | − | − | + | − | − | − | + | − | |
| A3 | − | − | − | − | − | − | − | − | − | − | + | − | |
| A4 | − | − | − | − | − | − | − | − | − | − | + | − | |
| A5 | − | − | − | − | − | − | + | + | − | − | + | + | |
| A6 | − | − | − | − | − | − | − | − | − | − | + | − | |
| Modified ΔV2 | A7 | + | + | − | − | − | − | + | + | + | + | + | − |
| A8 | + | + | − | − | − | − | + | + | + | + | + | + | |
| A9 | + | − | + | − | − | − | + | + | + | + | + | + | |
| A10 | − | − | + | + | − | − | + | + | − | − | + | + | |
| A11 | − | − | − | − | − | − | − | − | − | − | − | − | |
| A12 | − | − | − | − | − | − | − | − | − | − | − | − | |
a
Neutralizing activity was evaluated at 1:10 dilution, taking into consideration the nonspecific neutralization recorded with sera collected from animals vaccinated with the DNA vector alone (see Materials and Methods for details). −, 50% specific neutralization was not recorded; +, 50 or 80% specific neutralization was recorded. Results are from three independent neutralization experiments.