TABLE 2.
Neutralization of heterologous primary HIV-1 isolates by macaque sera
| Isolate (coreceptor usage) | MAb
|
% Neutralizationa with immunogenb
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| ΔV2 gp140
|
SF162 gp140
|
SF2 gp120
|
||||||||
| 2F5 | 2G12 | J408
|
H445
|
P655 (A) | N472 (A) | L714 | L814 | |||
| A | B | A | B | |||||||
| 91US056(R5) | 60 | 70 | 90 | — | 65 | — | — | — | — | — |
| 92US714(R5) | 70 | 20 | 85 | — | 85 | — | — | — | — | — |
| 92US660(R5) | 75 | 70 | 50 | — | 80 | — | — | — | — | — |
| 92HT593(R5X4) | 75 | 80 | — | — | — | — | — | — | — | — |
| 92US657(R5) | NT | NT | — | — | — | 65 | — | — | — | — |
| BZ167(R5X4) | 90 | 75 | NT | — | NT | 80 | NT | NT | NT | NT |
| ADA(R5) | NT | NT | 90 | 50 | 90 | 80 | NT | NT | NT | NT |
Percent neutralization of a given HIV-1 isolate by sera (1:10 dilution) collected from animals immunized with the modified ΔV2 gp140 (J408 and H445), unmodified SF162 gp140 (P655 and N472), and recombinant SF2 gp120 (L714 and L814), calculated as described in Materials and Methods, taking into consideration the nonspecific neutralization recorded with sera collected from the same animals before immunization.
A, sera collected 2 weeks following the DNA-plus-protein booster immunization; B, sera collected 2 weeks following the final protein booster immunization of animals J408 and H445. Values represent averages from two to three independent experiments. The susceptibility of these isolates to neutralization by 2F5 and 2G12 at 25 μg of MAb per ml is also presented. —, not detected; NT, not tested.